recipes


 * ACK Lysis Buffer (1x)**

8.29 g NH4Cl 1 g KHCO3 37.2 mg EDTA (Or 254ul from 500mM EDTA)

Total volume = 1 L, pH 7.2-7.4


 * LB**


 * 10 g NaCl **
 * 10 g tryptone **
 * 5 g yeast extract **


 * Total volume = 1 L, pH 7.0 **


 * LB Agar**


 * 10 g NaCl **
 * 10 g tryptone **
 * 5 g yeast extract **
 * 15 g agar **


 * Total volume = 1 L, pH 7.0 **


 * PBS (10x)**


 * 800 g NaCl **
 * 20 g KCl **
 * 144 g Na2HPO4 **
 * 24 g KH2PO4 **


 * Total volume = 10 L, pH 7.4 **


 * PCR Buffer (10x)**


 * 200 mM Tris **
 * 500 mM KCl **


 * pH 8.4 **


 * TAE (50x)**


 * 242 g Tris **
 * 100 ml 0.5 M EDTA **
 * 57 ml glacial acetic acid **


 * Total volume = 1 L, pH 8.5 **


 * Tail Lysis Buffer**